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Addgene

Lenti-dCas9-TurboID-blast
(Plasmid #222599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lenti-dCas9-KRAB-blast
  • Modifications to backbone
    Replace KRAB with TurboID
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-TurboID
  • Insert Size (bp)
    5175
  • Promoter EF-1α

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-dCas9-TurboID-blast was a gift from Yarui Diao (Addgene plasmid # 222599 ; http://n2t.net/addgene:222599 ; RRID:Addgene_222599)

  • For your References section:

    Crosstalk between RNA m(6)A and DNA methylation regulates transposable element chromatin activation and cell fate in human pluripotent stem cells. Sun T, Xu Y, Xiang Y, Ou J, Soderblom EJ, Diao Y. Nat Genet. 2023 Aug;55(8):1324-1335. doi: 10.1038/s41588-023-01452-5. Epub 2023 Jul 20. 10.1038/s41588-023-01452-5 PubMed 37474847
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