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Addgene

pSF503
(Plasmid #222587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMST635_BB3_L_AC_hyg_KanOri_pyrG5
  • Backbone manufacturer
    Michael Sauer (Sarkari et al. 2017) Addgene #90280
  • Total vector size (bp) 15636
  • Vector type
    Bacterial Expression ; A. niger
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gsdA
  • Insert Size (bp)
    1516
  • Mutation
    Internal BsaI and BpiI sites removed from insert, PAM site for gRNA1(gsdA) mutated
  • Entrez Gene
    gsdA (a.k.a. An02g12140)
  • Promoter tet-on

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer TCATGAATCATGCGGCCGCGCGTATCACGAGGCCCTTTCGACTTCACTCGAGTTTACCA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF503 was a gift from Matthias Steiger (Addgene plasmid # 222587 ; http://n2t.net/addgene:222587 ; RRID:Addgene_222587)

  • For your References section:

    Regulation of the Glucose-6-phosphate dehydrogenase encoding gene gsdA and its impact on growth and citric acid production in Aspergillus niger. Matthias Steiger. PLOS One 10.1371/journal.pone.0321363
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