Skip to main content
Addgene

pPB-cT3G-cERH-HOXB5
(Plasmid #222584)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222584 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPB-cT3G-cERH
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB5
  • Species
    H. sapiens (human)
  • Entrez Gene
    HOXB5 (a.k.a. HHO.C10, HOX2, HOX2A, HU-1, Hox2.1)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is an amino acid substitution in the P2A linker. Depositor confirms this does not affect HOXB5 expression and hygromycin resistance, but notes that the cleavage efficiency may be reduced.

Please visit https://doi.org/10.1101/2024.05.31.596483 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-cT3G-cERH-HOXB5 was a gift from George Church (Addgene plasmid # 222584 ; http://n2t.net/addgene:222584 ; RRID:Addgene_222584)

  • For your References section:

    Induction of Meiosis from Human Pluripotent Stem Cells. Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, Church GM. bioRxiv 2024.05.31.596483 10.1101/2024.05.31.596483
Related items: