pENTR/shp16 (w98-1)
(Plasmid
#22258)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22258 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR1A
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2920
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Vector typeRNAi ; Entry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesh cyclin-dependent kinase inhibitor 2A
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Alt nameshp16
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gRNA/shRNA sequencenone given
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SpeciesH. sapiens (human)
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Insert Size (bp)61
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Entrez GeneCDKN2A (a.k.a. ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho I (destroyed during cloning)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byshRNA obtained from Dr. Christian Beausejour.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR/shp16 (w98-1) was a gift from Eric Campeau (Addgene plasmid # 22258 ; http://n2t.net/addgene:22258 ; RRID:Addgene_22258)
Map uploaded by the depositor.
