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pPB-cT3G-cERP2-3xFLAG-SMAD1-active
(Plasmid #222579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB-cT3G-cERP2
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMAD1
  • Species
    H. sapiens (human)
  • Mutation
    S487E, S489E
  • Entrez Gene
    SMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.31.596483 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-cT3G-cERP2-3xFLAG-SMAD1-active was a gift from George Church (Addgene plasmid # 222579 ; http://n2t.net/addgene:222579 ; RRID:Addgene_222579)

  • For your References section:

    Induction of Meiosis from Human Pluripotent Stem Cells. Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, Church GM. bioRxiv 2024.05.31.596483 10.1101/2024.05.31.596483
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