pLenti CMV GSE T329A Hygro (w189-4)
(Plasmid #22255)

• Depositing Lab: Eric Campeau
• Publication: shMDC1 plasmids (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 8712
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Genetic suppressor element #22, T329A
• Alt name: GS22 T329
• Species: R. norvegicus (rat)
• Insert Size (bp): 245
• Mutation: Threonine 329 is mutated to Alanine (p53 nomencature).

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: CMVforw
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GSE22 T329A was obtained from Dr. Christian Beausejour.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes amino acids #312-391 of rat p53 to act as a dominant-negative
(Ossovskaya et al. 1996, PNAS 93:10309-10314).

T329A mutation does not prevent tetramerization of p53
(Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).