pPB-cT3G-cERP2-CTCFL
(Plasmid
#222546)
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PurposePiggyBac transposon plasmid for doxycycline inducible expression of CTCFL
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPB-cT3G-cERP2
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCTCFL
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SpeciesH. sapiens (human)
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Entrez GeneCTCFL (a.k.a. BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.31.596483 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-cT3G-cERP2-CTCFL was a gift from George Church (Addgene plasmid # 222546 ; http://n2t.net/addgene:222546 ; RRID:Addgene_222546) -
For your References section:
Induction of Meiosis from Human Pluripotent Stem Cells. Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, Church GM. bioRxiv 2024.05.31.596483 10.1101/2024.05.31.596483