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E7 in pENTR1A (w186-1)
(Plasmid #22254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR1A
  • Backbone size w/o insert (bp) 2298
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human papilloma virus E7
  • Alt name
    E7
  • Insert Size (bp)
    296
  • Entrez Gene
    E7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Xho I (destroyed during cloning)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    E7 cDNA was obtained from Dr. Christian Beausejour.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The E7 protein binds and degrades the Retinoblastoma protein. It also binds to Mi2beta-HDAC1 from the NURD complex (Brehm et al. 1999).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E7 in pENTR1A (w186-1) was a gift from Eric Campeau (Addgene plasmid # 22254 ; http://n2t.net/addgene:22254 ; RRID:Addgene_22254)
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