GSE22 T329A in pENTR1A (w187-1)
(Plasmid #22253)

• Depositing Lab: Eric Campeau
• Publication: shMDC1 plasmids (unpublished)

Backbone

• Vector backbone: pENTR1A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2277
• Vector type: Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 370C.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Genetic suppressor element #22, T329A
• Alt name: GSE22 T329A
• Species: R. norvegicus (rat)
• Insert Size (bp): 245
• Mutation: Threonine 329 is mutated to Alanine (p53 nomencature).

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Xho I (destroyed during cloning)
• 5′ sequencing primer: ENTRforw
• 3′ sequencing primer: ENTRrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GSE22 was obtained from Dr.Christian Beausejour

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GSE22 originally published in Ossovskaya et al. PNAS 93: 10309-10314. T329A mutation was shown to not affect tetramerization of p53 (Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).