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pMX-Sox17-p2A-Erg-t2A-GFP
(Plasmid #222496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMX
  • Backbone size w/o insert (bp) 4582
  • Total vector size (bp) 8170
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Sox17
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1257
  • Entrez Gene
    Sox17

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Erg
  • Insert Size (bp)
    1459
  • Entrez Gene
    Erg (a.k.a. D030036I24Rik)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCGCCACAAACTTCTCTCTGCTAAAGCAAGCAGGTGATGTTGAAGAAAACCCCGGGCCTACTAGTATGATCCAGACTGTACCTGA
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    720

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX-Sox17-p2A-Erg-t2A-GFP was a gift from Li Qian (Addgene plasmid # 222496 ; http://n2t.net/addgene:222496 ; RRID:Addgene_222496)

  • For your References section:

    Direct conversion of cardiac fibroblasts into endothelial-like cells using Sox17 and Erg. Farber G, Dong Y, Wang Q, Rathod M, Wang H, Dixit M, Keepers B, Xie Y, Butz K, Polacheck WJ, Liu J, Qian L. Nat Commun. 2024 May 16;15(1):4170. doi: 10.1038/s41467-024-48354-6. 10.1038/s41467-024-48354-6 PubMed 38755186
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