pMX-Sox17-p2A-Erg-t2A-GFP
(Plasmid #222496)

• Purpose: mouse fibroblast-to-endothelial cell direct reprogramming
• Depositing Lab: Li Qian
• Publication: Farber et al Nat Commun. 2024 May 16;15(1):4170. doi: 10.1038/s41467-024-48354-6.

Backbone

• Vector backbone: pMX
• Backbone size w/o insert (bp): 4582
• Total vector size (bp): 8170
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Sox17
• Species: M. musculus (mouse)
• Insert Size (bp): 1257
• Entrez Gene: Sox17

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGCCGGATCTAGCTAGTTAATTAAATGAGCAGCCCGGATGCG

Gene/Insert 2

• Gene/Insert name: Erg
• Insert Size (bp): 1459
• Entrez Gene: Erg (a.k.a. D030036I24Rik)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCGCCACAAACTTCTCTCTGCTAAAGCAAGCAGGTGATGTTGAAGAAAACCCCGGGCCTACTAGTATGATCCAGACTGTACCTGA

Gene/Insert 3

• Gene/Insert name: EGFP
• Insert Size (bp): 720

Resource Information

• Supplemental Documents:
  • SEG_Sox-SEG_P1.gb
  • SEG_Erg-SEG_P3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry