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Addgene

pESC-HIS (V5 and HA)
(Plasmid #222487)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pESC-URA
  • Backbone manufacturer
    Agilent Technologies
  • Backbone size (bp) 6727
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • HA (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • HA (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.05.01.538886 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pESC-HIS (V5 and HA) was a gift from Tetsuo Matsuzaki & Kiyofumi Yamada (Addgene plasmid # 222487 ; http://n2t.net/addgene:222487 ; RRID:Addgene_222487)

  • For your References section:

    A novel plasmid-based experimental system in Saccharomyces cerevisiae that enables the introduction of 10 different plasmids into cells. Dong G, Nakai T, Matsuzaki T. FEBS Open Bio. 2024 Oct 10. doi: 10.1002/2211-5463.13893. 10.1002/2211-5463.13893 PubMed 39387713
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