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Addgene

pMpGWBs02
(Plasmid #222478)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222478 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMpGWBs00
  • Modifications to backbone
    Restriction cloning of a "35s promoter and GATEWAY cassette" from pMpGWB102 into the pMpGWBs00 backbone via the HindIII/SacI sites.
  • Vector type
    Plant Expression
  • Selectable markers
    Sulfadiazine

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGWBs02 was a gift from Philip Carella (Addgene plasmid # 222478 ; http://n2t.net/addgene:222478 ; RRID:Addgene_222478)

  • For your References section:

    An Efficient Sulfadiazine Selection Scheme for Stable Transformation in the Model Liverwort Marchantia polymorpha. Robinson K, Chia KS, Guyon A, Schornack S, Carella P. J Exp Bot. 2024 Jun 2:erae256. doi: 10.1093/jxb/erae256. 10.1093/jxb/erae256 PubMed 38824404
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