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Addgene

pMpGWBs00
(Plasmid #222476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMpGWB100
  • Backbone manufacturer
    Takayuki Kohchi, Kyoto University
  • Modifications to backbone
    We replaced the hygromycin resistance gene for a sulfonamide resistant DHPS enzyme (tp-SulR) to enable sulfadiazine selection.
  • Vector type
    Plant Expression
  • Selectable markers
    Sulfadiazine

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGWBs00 was a gift from Philip Carella (Addgene plasmid # 222476 ; http://n2t.net/addgene:222476 ; RRID:Addgene_222476)

  • For your References section:

    An Efficient Sulfadiazine Selection Scheme for Stable Transformation in the Model Liverwort Marchantia polymorpha. Robinson K, Chia KS, Guyon A, Schornack S, Carella P. J Exp Bot. 2024 Jun 2:erae256. doi: 10.1093/jxb/erae256. 10.1093/jxb/erae256 PubMed 38824404
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