pGL4.23-hs737.3
(Plasmid
#222475)
-
PurposeLuciferase vector containing the hs737 enhancer sequence (sequence containing variant 3). Variant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 222475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL4.23
-
Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4279
- Total vector size (bp) 5417
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehs737 enhancer sequence (sequence containing variant 3)
-
Alt nameairplane enhancer
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1138
-
MutationVariant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.
-
Entrez GeneLOC110120928
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL4.23-hs737.3 was a gift from Tychele Turner (Addgene plasmid # 222475 ; http://n2t.net/addgene:222475 ; RRID:Addgene_222475) -
For your References section:
Coding and noncoding variants in EBF3 are involved in HADDS and simplex autism. Padhi EM, Hayeck TJ, Cheng Z, Chatterjee S, Mannion BJ, Byrska-Bishop M, Willems M, Pinson L, Redon S, Benech C, Uguen K, Audebert-Bellanger S, Le Marechal C, Ferec C, Efthymiou S, Rahman F, Maqbool S, Maroofian R, Houlden H, Musunuri R, Narzisi G, Abhyankar A, Hunter RD, Akiyama J, Fries LE, Ng JK, Mehinovic E, Stong N, Allen AS, Dickel DE, Bernier RA, Gorkin DU, Pennacchio LA, Zody MC, Turner TN. Hum Genomics. 2021 Jul 13;15(1):44. doi: 10.1186/s40246-021-00342-3. 10.1186/s40246-021-00342-3 PubMed 34256850