pFast-hHus1
(Plasmid
#222469)
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PurposeExpresses hHus1 in insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4775
- Total vector size (bp) 7782
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFast-hHus1 was a gift from Aziz Sancar (Addgene plasmid # 222469 ; http://n2t.net/addgene:222469 ; RRID:Addgene_222469) -
For your References section:
Purification and characterization of human DNA damage checkpoint Rad complexes. Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11236-41. doi: 10.1073/pnas.201373498. 10.1073/pnas.201373498 PubMed 11572977