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Addgene

pFast-His6-hRad17
(Plasmid #222465)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222465 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBacHTa
  • Backbone manufacturer
    Gibco/BRL
  • Backbone size w/o insert (bp) 4856
  • Total vector size (bp) 7910
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hRad17
  • Alt name
    Human cell cycle checkpoint protein Rad 17
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3054
  • Entrez Gene
    RAD17 (a.k.a. CCYC, HRAD17, R24L, RAD17SP, RAD24)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (unknown if destroyed)
  • 3′ cloning site Eco RI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    hRad17 was amplified by PCR with pACT2-hRad17, a kind gift of Jorge Vialard (Janssen Research Foundation, Beerse, Belgium), as the template.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFast-His6-hRad17 was a gift from Aziz Sancar (Addgene plasmid # 222465 ; http://n2t.net/addgene:222465 ; RRID:Addgene_222465)

  • For your References section:

    Purification and characterization of human DNA damage checkpoint Rad complexes. Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A. Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11236-41. doi: 10.1073/pnas.201373498. 10.1073/pnas.201373498 PubMed 11572977
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