pCHW00014
(Plasmid
#222445)
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PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222445 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57 (kan)
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Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVP16-NLS
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SpeciesSynthetic
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Insert Size (bp)258
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Mutationno
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Overhangs: TTCG-GCTT.
Please visit https://doi.org/10.1101/2024.03.23.586378 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCHW00014 was a gift from Chih-Hang Wu (Addgene plasmid # 222445 ; http://n2t.net/addgene:222445 ; RRID:Addgene_222445) -
For your References section:
Development of a tightly regulated copper-inducible transient gene expression system in Nicotiana benthamiana incorporating suicide exon and Cre recombinase. Chiang BJ, Lin KY, Chen YF, Huang CY, Goh FJ, Huang LT, Chen LH, Wu CH. bioRxiv 2024.03.23.586378 10.1101/2024.03.23.586378