pGGB_YFP-MiniTurboID
(Plasmid #222433)

• Purpose: Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
• Depositing Lab: Dominique Bergmann
• Publication: Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5. doi: 10.1016/j.devcel.2024.03.001. Epub 2024 Mar 21.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 4290
• Vector type: Golden Gate / Green Gate compatible cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MiniTurboID (BirA mutant)
• Species: BirA from E. coli, codon optimized for human and mutated
• Insert Size (bp): 771
• Mutation: aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
• Tags / Fusion Proteins:
  • YFP (N terminal on insert)
  • Linker (N terminal on insert)
  • Linker (C terminal on insert)

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • pGGB_YFP-MiniTurboID.gb
• A portion of this plasmid was derived from a plasmid made by: Alice Ting's lab at Stanford University (MiniTurbo cloned from V5-miniTurbo-NES_pCDNA3, Addgene catalog #107170).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry