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pGGI_Myr-YFP-MiniTurboID
(Plasmid #222432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222432 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4312
  • Vector type
    Golden Gate / Green Gate compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MiniTurboID (BirA mutant)
  • Species
    BirA from E. coli, codon optimized for human and mutated
  • Insert Size (bp)
    771
  • Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • Linker (N terminal on insert)
    • Myr (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Alice Ting's lab at Stanford University (MiniTurbo cloned from V5-miniTurbo-NES_pCDNA3, Addgene #107170).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Myristoylation tag designed to bring protein to plasma membrane, but some fusion protein detected in all membranes in Arabidopsis cells. Not used in paper.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGI_Myr-YFP-MiniTurboID was a gift from Dominique Bergmann (Addgene plasmid # 222432 ; http://n2t.net/addgene:222432 ; RRID:Addgene_222432)

  • For your References section:

    Spatially resolved proteomics of the Arabidopsis stomatal lineage identifies polarity complexes for cell divisions and stomatal pores. Wallner ES, Mair A, Handler D, McWhite C, Xu SL, Dolan L, Bergmann DC. Dev Cell. 2024 May 6;59(9):1096-1109.e5. doi: 10.1016/j.devcel.2024.03.001. Epub 2024 Mar 21. 10.1016/j.devcel.2024.03.001 PubMed 38518768
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