pcDNA3-Golgi-deltaCKAR
(Plasmid #222428)

• Purpose: FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.
• Depositing Lab: Alexandra Newton
• Publication: Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-910.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5292
• Total vector size (bp): 7499
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Golgi delta-selective C kinase activity reporter
• Alt name: Golgi-dCKAR
• Species: Synthetic
• Insert Size (bp): 2207
• Tags / Fusion Proteins:
  • CFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Supplemental Documents:
  • SKW5Y3_3_Golgi_dCKAR_2.11.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry