LBR(N547D)-FKBP-GFP
(Plasmid
#222422)
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PurposeTo express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLBR-FKBP-GFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLBR(N547D)
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SpeciesH. sapiens (human)
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MutationN547D
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Entrez GeneLBR (a.k.a. C14SR, DHCR14B, LMN2R, PHA, PHASK, TDRD18)
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Tag
/ Fusion Protein
- FKBP-GFP (C terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.31.596797 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LBR(N547D)-FKBP-GFP was a gift from Stephen Royle (Addgene plasmid # 222422 ; http://n2t.net/addgene:222422 ; RRID:Addgene_222422) -
For your References section:
Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor. Downie L, Ferrandiz N, Megan Jones M, Royle SJ. bioRxiv 2024.05.31.596797 10.1101/2024.05.31.596797
