Skip to main content
Addgene

pEGFPC1-EPSIN 2
(Plasmid #22242)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epsin 2
  • Alt name
    EH domain-binding protein
  • Alt name
    EHB21
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • GenBank ID
    AF062085
  • Entrez Gene
    EPN2 (a.k.a. EHB21)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer
  • 3′ sequencing primer EGFP-C Sequencing Primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-EPSIN 2 was a gift from Pietro De Camilli (Addgene plasmid # 22242 ; http://n2t.net/addgene:22242 ; RRID:Addgene_22242)

  • For your References section:

    The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module. Rosenthal JA, Chen H, Slepnev VI, Pellegrini L, Salcini AE, Di Fiore PP, De Camilli P. J Biol Chem. 1999 Nov 26. 274(48):33959-65. 10.1074/jbc.274.48.33959 PubMed 10567358
Related items:
Commonly requested with: