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Addgene

Stargazin-EBFP2-FRB
(Plasmid #222417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FRB-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cacng2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cacng2 (a.k.a. B230105C07Rik, B930041E13Rik, stargazer, stargazin, stg, wag, waggler)
  • Tag / Fusion Protein
    • EBFP2-HA-FRB (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.31.596797 for bioRxiv preprint.

There are two discrepancies in the Addgene sequence file compared to the canonical sequence: F325L mutation in stargazin region of stargazin-EBFP2-HA-FRB ORF, as well as a F416L mutation in EBFP2 region of stargazin-EBFP2-HA-FRB ORF. The authors confirm these mutations do not have an effect on the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stargazin-EBFP2-FRB was a gift from Stephen Royle (Addgene plasmid # 222417 ; http://n2t.net/addgene:222417 ; RRID:Addgene_222417)

  • For your References section:

    Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor. Downie L, Ferrandiz N, Megan Jones M, Royle SJ. bioRxiv 2024.05.31.596797 10.1101/2024.05.31.596797
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