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Addgene

pSS2 - pBait-1xMS2-7GC[empty]-TtrpA
(Plasmid #222406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF
  • Backbone size (bp) 3945
  • Modifications to backbone
    Relative to pCH1, this construct contains an additional 7bp GC clamp surrounding the XmaI and HindIII restriction sites, as well as the intrinsic terminator from E. coli trpA downstream of HindIII.
  • Vector type
    Bacterial Expression
  • Promoter pBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSS2 - pBait-1xMS2-7GC[empty]-TtrpA was a gift from Katherine Berry (Addgene plasmid # 222406 ; http://n2t.net/addgene:222406 ; RRID:Addgene_222406)

  • For your References section:

    Improved constructs for bait RNA display in a bacterial three-hybrid assay. Linh D. Nguyen , Hannah LeBlanc & Katherine E. Berry. Sci Rep 15, 3820 (2025) 10.1038/s41598-024-85082-9
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