pSS1 - pBait-5GC[1xMS2]-empty
(Plasmid
#222405)
-
Purpose(Empty Backbone) arabinose-induced synthesis of an RNA containing an MS2 hairpin flanked by a 5GC clamp. Used as a three-hybrid control. For cloning purposes, see plasmid #222403.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCDF
- Backbone size (bp) 3901
-
Modifications to backboneRelative to pCH1, this construct contains an additional 5bp GC clamp surrounding the MS2 hairpin.
-
Vector typeBacterial Expression
- Promoter pBAD
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSS1 - pBait-5GC[1xMS2]-empty was a gift from Katherine Berry (Addgene plasmid # 222405 ; http://n2t.net/addgene:222405 ; RRID:Addgene_222405) -
For your References section:
Improved constructs for bait RNA display in a bacterial three-hybrid assay. Linh D. Nguyen , Hannah LeBlanc & Katherine E. Berry. Sci Rep 15, 3820 (2025) 10.1038/s41598-024-85082-9
