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Purpose2nd generation lentiviral vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 6655
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namevexGFP_2A_OCT4
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Alt nameOct4
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SpeciesH. sapiens (human); synthetic fluorescent protein
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Insert Size (bp)1872
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer hPGK-F (5'GTGTTCCGCATTCTGCAAG-3')
- 3′ sequencing primer WPRE-R (5'-CATAGCGTAAAAGGAGCAACA-3') (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLM-vexGFP-Oct4 was a gift from Michel Sadelain (Addgene plasmid # 22240 ; http://n2t.net/addgene:22240 ; RRID:Addgene_22240) -
For your References section:
Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64. 10.1073/pnas.0904825106 PubMed 19549847