pAAV2-CAGIG-LAMP1-mGL
(Plasmid
#222394)
-
PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAAV2-CAGIG
-
Backbone manufacturerPantelis Tsoulfas
- Backbone size w/o insert (bp) 4711
- Total vector size (bp) 6724
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameLAMP1 and mGreenlantern
-
SpeciesH. sapiens (human), Synthetic; Homo Sapiens
-
Insert Size (bp)2013
-
GenBank IDNP_005552.3
-
Entrez GeneLAMP1 (a.k.a. CD107a, LAMPA, LGP120)
- Promoter CAG or CAGIG
-
Tags
/ Fusion Proteins
- LAMP1 (N terminal on insert)
- mGreenLantern (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer pCACGGS-5
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV2-CAGIG-LAMP1-mGL was a gift from Pantelis Tsoulfas (Addgene plasmid # 222394 ; http://n2t.net/addgene:222394 ; RRID:Addgene_222394)
Map uploaded by the depositor.
