pAAV2-CAGIG-LAMP1-mGL
(Plasmid #222394)

• Purpose: To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.
• Depositing Lab: Pantelis Tsoulfas
• Publication: Actin-Endosomes (unpublished)

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Backbone

• Vector backbone: pAAV2-CAGIG
• Backbone manufacturer: Pantelis Tsoulfas
• Backbone size w/o insert (bp): 4711
• Total vector size (bp): 6724
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAMP1 and mGreenlantern
• Species: H. sapiens (human), Synthetic; Homo Sapiens
• Insert Size (bp): 2013
• GenBank ID: NP_005552.3
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Promoter: CAG or CAGIG
• Tags / Fusion Proteins:
  • LAMP1 (N terminal on insert)
  • mGreenLantern (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: pCACGGS-5
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry