pLV-EGFP-Human-UTROPHIN_1-261
(Plasmid #222393)

• Purpose: Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
• Depositing Lab: Pantelis Tsoulfas
• Publication: Actin-Endosomes (unpublished)

Backbone

• Vector backbone: pLenti-MP2
• Backbone manufacturer: Pantelis Tsoulfas
• Backbone size w/o insert (bp): 6698
• Total vector size (bp): 8210
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human Utrophin residues 1-261 and EGFP
• Alt name: dystrophin-related protein
• Species: H. sapiens (human); Homo Sapiens
• Insert Size (bp): 1512
• Mutation: Sequence is derived from ADDGENE Plasmid #26737-GFP
• GenBank ID: X69086
• Entrez Gene: UTRN (a.k.a. DMDL, DRP, DRP1)
• Promoter: CMV promoter and enhancer
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • Human UTROPHIN 1-261 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: LCNX
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry