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pAF146
(Plasmid #222377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAF112
  • Backbone manufacturer
    Andrew S. Flies Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin ; Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDL1
  • Alt name
    CD274 (programmed cell death 1 ligand 1)
  • Species
    Tasmanian devil (Sarcophilus harrisii)
  • Promoter EF1a

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid generated by Gibson assembly using NotI and SmaI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAF146 was a gift from Andrew S. Flies (Addgene plasmid # 222377 ; http://n2t.net/addgene:222377 ; RRID:Addgene_222377)

  • For your References section:

    [Agammaglobulinemia and hypogammaglobulinemia in newborn lambs depending on the time of the first suckling]. Georgiev S. Vet Med Nauki. 1978;15(4):93-8. PubMed 84431
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