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Addgene

peCALWY-5
(Plasmid #22237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22237 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV (Clontech)
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E.coli nova blue LB culture medium 30 ug/ml kanamycin Culture overnight, 37 C/250 rpm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eCALWY-5 Zn(II) sensor
  • Species
    H. sapiens (human); GFP's
  • Insert Size (bp)
    2100
  • Mutation
    C416S mutation identical to peCALWY-4, but linker consists of 5 GGSGGS repeats
  • Entrez Gene
    ATOX1 (a.k.a. ATX1, HAH1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV promotor
  • 3′ sequencing primer CMV terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peCALWY-5 was a gift from Maarten Merkx (Addgene plasmid # 22237 ; http://n2t.net/addgene:22237 ; RRID:Addgene_22237)

  • For your References section:

    Genetically encoded FRET sensors to monitor intracellular Zn(2+) homeostasis. Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M. Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368. 10.1038/nmeth.1368 PubMed 19718032
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