pJMP12049
(Plasmid
#222355)
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PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneR6Kγ
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Vector typeBacterial Expression
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BW25141
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesDerived from Aequorea victoria.
- Promoter PabstBR
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Tn7 expression vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJMP12049 was a gift from Jason Peters (Addgene plasmid # 222355 ; http://n2t.net/addgene:222355 ; RRID:Addgene_222355)