pJMP3654
(Plasmid
#222349)
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PurposeExpression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15A and pWH1266
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesDerived from Aequorea victoria.
- Promoter PabstBR
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Shuttle vector that replicates in A. baumannii and E. coli; replicates in common cloning strains such as DH10B.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJMP3654 was a gift from Jason Peters (Addgene plasmid # 222349 ; http://n2t.net/addgene:222349 ; RRID:Addgene_222349)
