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Addgene

AAVS1 pegRNA1 with trimmed attP
(Plasmid #222346)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222346 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pU6
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAVS1 attP-installation pegRNA1
  • Species
    Synthetic

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1 pegRNA1 with trimmed attP was a gift from David Liu (Addgene plasmid # 222346 ; http://n2t.net/addgene:222346 ; RRID:Addgene_222346)

  • For your References section:

    Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing. Pandey S, Gao XD, Krasnow NA, McElroy A, Tao YA, Duby JE, Steinbeck BJ, McCreary J, Pierce SE, Tolar J, Meissner TB, Chaikof EL, Osborn MJ, Liu DR. Nat Biomed Eng. 2024 Jun 10. doi: 10.1038/s41551-024-01227-1. 10.1038/s41551-024-01227-1 PubMed 38858586
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