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Addgene

eeBxb1_IVT
(Plasmid #222342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7
  • Modifications to backbone
    Inactivated T7 promoter to prevent transcription from plasmid
  • Vector type
    Template for in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimized evolved and engineered Bxb1
  • Species
    Synthetic
  • Promoter T7 (inactivated)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eeBxb1_IVT was a gift from David Liu (Addgene plasmid # 222342 ; http://n2t.net/addgene:222342 ; RRID:Addgene_222342)

  • For your References section:

    Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing. Pandey S, Gao XD, Krasnow NA, McElroy A, Tao YA, Duby JE, Steinbeck BJ, McCreary J, Pierce SE, Tolar J, Meissner TB, Chaikof EL, Osborn MJ, Liu DR. Nat Biomed Eng. 2024 Jun 10. doi: 10.1038/s41551-024-01227-1. 10.1038/s41551-024-01227-1 PubMed 38858586
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