wtBxb1_IVT
(Plasmid
#222340)
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PurposePlasmid for wild type Bxb1 mRNA in vitro transcription template
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 222340 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepT7
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Modifications to backboneInactivated T7 promoter to prevent transcription from plasmid
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Vector typeTemplate for in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCodon-optimized wtBxb1
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SpeciesSynthetic
- Promoter T7 (inactivated)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
wtBxb1_IVT was a gift from David Liu (Addgene plasmid # 222340 ; http://n2t.net/addgene:222340 ; RRID:Addgene_222340) -
For your References section:
Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing. Pandey S, Gao XD, Krasnow NA, McElroy A, Tao YA, Duby JE, Steinbeck BJ, McCreary J, Pierce SE, Tolar J, Meissner TB, Chaikof EL, Osborn MJ, Liu DR. Nat Biomed Eng. 2024 Jun 10. doi: 10.1038/s41551-024-01227-1. 10.1038/s41551-024-01227-1 PubMed 38858586
Map uploaded by the depositor.
