pFlp-Km
(Plasmid #222334)

• Purpose: flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3
• Depositing Lab: Philip Poole
• Publication: Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2.

Backbone

• Vector backbone: pBBR1-Km-ELT3 (pOGG023)
• Backbone manufacturer: Philip Poole lab
• Backbone size w/o insert (bp): 3540
• Total vector size (bp): 7177
• Modifications to backbone: Gene sacB-flp-cI were amplified from pFLP2 and cloned into a pBBR1-Km plasmid (p0GG023) using Golden Gate

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: sacB
• Alt name: levansucrase
• Species: B. subtilis
• Entrez Gene: sacB (a.k.a. BSU_34450, BSU34450)
• Promoter: sacB

Cloning Information for Gene/Insert 1

• Cloning method: Golden Gate
• 5′ sequencing primer: Golden Gate Assembly

Gene/Insert 2

• Gene/Insert name: flp
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: flp
• Promoter: flp

Cloning Information for Gene/Insert 2

• Cloning method: Golden Gate
• 5′ sequencing primer: Golden Gate Assembly

Gene/Insert 3

• Gene/Insert name: cI
• Alt name: lambda represor
• Entrez Gene: cI (a.k.a. AVT79_gp39)
• Promoter: cl

Cloning Information for Gene/Insert 3

• Cloning method: Golden Gate
• 5′ sequencing primer: Golden Gate assembly

Resource Information

• Supplemental Documents:
  • pFlp-Km.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry