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pFlp-Km
(Plasmid #222334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1-Km-ELT3 (pOGG023)
  • Backbone manufacturer
    Philip Poole lab
  • Backbone size w/o insert (bp) 3540
  • Total vector size (bp) 7177
  • Modifications to backbone
    Gene sacB-flp-cI were amplified from pFLP2 and cloned into a pBBR1-Km plasmid (p0GG023) using Golden Gate

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sacB
  • Alt name
    levansucrase
  • Species
    B. subtilis
  • Entrez Gene
    sacB (a.k.a. BSU_34450, BSU34450)
  • Promoter sacB

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    flp
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    flp
  • Promoter flp

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    cI
  • Alt name
    lambda represor
  • Entrez Gene
    cI (a.k.a. AVT79_gp39)
  • Promoter cl

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlp-Km was a gift from Philip Poole (Addgene plasmid # 222334 ; http://n2t.net/addgene:222334 ; RRID:Addgene_222334)

  • For your References section:

    Stable, fluorescent markers for tracking synthetic communities and assembly dynamics. Jorrin B, Haskett TL, Knights HE, Martyn A, Underwood TJ, Dolliver J, Ledermann R, Poole PS. Microbiome. 2024 May 7;12(1):81. doi: 10.1186/s40168-024-01792-2. 10.1186/s40168-024-01792-2 PubMed 38715147
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