CAP-AAV.FUS.3
(Plasmid
#222333)
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PurposeAAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-Amp
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
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SpeciesSynthetic
- Promoter p41
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsiWI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAP-AAV.FUS.3 was a gift from Mikhail Shapiro & Jerzy Szablowski (Addgene plasmid # 222333 ; http://n2t.net/addgene:222333 ; RRID:Addgene_222333) -
For your References section:
Engineering viral vectors for acoustically targeted gene delivery. Li HR, Harb M, Heath JE, Trippett JS, Shapiro MG, Szablowski JO. Nat Commun. 2024 Jun 10;15(1):4924. doi: 10.1038/s41467-024-48974-y. 10.1038/s41467-024-48974-y PubMed 38858354