Str-KDEL_CD9-SBP-mCherry
(Plasmid
#222329)
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PurposeSynchronize the trafficking of CD9 from the ER.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES-neo3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStreptavidin-KDEL and CD9 fused to SBP-mCherry
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Alt nameTSPAN-29
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SpeciesH. sapiens (human)
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Entrez GeneCD9 (a.k.a. BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Str-KDEL_CD9-SBP-mCherry was a gift from Franck Perez (Addgene plasmid # 222329 ; http://n2t.net/addgene:222329 ; RRID:Addgene_222329) -
For your References section:
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Mathieu M, Nevo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Thery C. Nat Commun. 2021 Jul 19;12(1):4389. doi: 10.1038/s41467-021-24384-2. 10.1038/s41467-021-24384-2 PubMed 34282141