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Addgene

Str-KDEL_CD63-SBP-mCherry
(Plasmid #222326)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222326 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES-neo3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-KDEL and CD63 fused to SBP-mCherry
  • Species
    H. sapiens (human)
  • Entrez Gene
    CD63 (a.k.a. AD1, HOP-26, ME491, MLA1, OMA81H, Pltgp40, TSPAN30)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_CD63-SBP-mCherry was a gift from Franck Perez (Addgene plasmid # 222326 ; http://n2t.net/addgene:222326 ; RRID:Addgene_222326)
  • For your References section:

    Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Mathieu M, Nevo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Thery C. Nat Commun. 2021 Jul 19;12(1):4389. doi: 10.1038/s41467-021-24384-2. 10.1038/s41467-021-24384-2 PubMed 34282141