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Str-KDEL_SBP-EGFP-EGFR
(Plasmid #222325)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222325 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-neo3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-KDEL and EGFR fused to SBP-EGFP
  • Alt name
    HER1
  • Alt name
    EGFR
  • Species
    H. sapiens (human)
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_SBP-EGFP-EGFR was a gift from Franck Perez (Addgene plasmid # 222325 ; http://n2t.net/addgene:222325 ; RRID:Addgene_222325)

  • For your References section:

    The endosomal transcriptional regulator RNF11 integrates degradation and transport of EGFR. Scharaw S, Iskar M, Ori A, Boncompain G, Laketa V, Poser I, Lundberg E, Perez F, Beck M, Bork P, Pepperkok R. J Cell Biol. 2016 Nov 21;215(4):543-558. doi: 10.1083/jcb.201601090. Epub 2016 Nov 8. 10.1083/jcb.201601090 PubMed 27872256
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