Str-KDEL_SBP-EGFP-LAP
(Plasmid
#222322)
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PurposeSynchronize the trafficking of LAP from the ER.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES-neo3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStreptavidin-KDEL and LAP fused to SBP-EGFP
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Alt nameACP2
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Alt nameLAP
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SpeciesH. sapiens (human)
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Entrez GeneACP2 (a.k.a. LAP)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Str-KDEL_SBP-EGFP-LAP was a gift from Franck Perez (Addgene plasmid # 222322 ; http://n2t.net/addgene:222322 ; RRID:Addgene_222322) -
For your References section:
Lysosomal membrane proteins LAMP1 and LIMP2 are segregated in the Golgi apparatus independently of their clathrin adaptor binding motif. Ecard J, Lian YL, Divoux S, Gouveia Z, Vigne E, Perez F, Boncompain G. Mol Biol Cell. 2024 Mar 1;35(3):ar42. doi: 10.1091/mbc.E23-06-0251. Epub 2024 Jan 17. 10.1091/mbc.E23-06-0251 PubMed 38231876