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Str-KDEL_SBP-EGFP-LAMP2
(Plasmid #222321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222321 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-neo3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-KDEL and LAMP2 fused to SBP-EGFP
  • Alt name
    LAMP2B
  • Species
    H. sapiens (human)
  • Entrez Gene
    LAMP2 (a.k.a. CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_SBP-EGFP-LAMP2 was a gift from Franck Perez (Addgene plasmid # 222321 ; http://n2t.net/addgene:222321 ; RRID:Addgene_222321)

  • For your References section:

    Lysosomal membrane proteins LAMP1 and LIMP2 are segregated in the Golgi apparatus independently of their clathrin adaptor binding motif. Ecard J, Lian YL, Divoux S, Gouveia Z, Vigne E, Perez F, Boncompain G. Mol Biol Cell. 2024 Mar 1;35(3):ar42. doi: 10.1091/mbc.E23-06-0251. Epub 2024 Jan 17. 10.1091/mbc.E23-06-0251 PubMed 38231876
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