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Addgene

pCMV Flag dPDZ-PTEN (1-400)
(Plasmid #22232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag2
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTEN
  • Alt name
    PTEN
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Mutation
    PDZ binding domain deleted. Contains amino acids 1-400.
  • Entrez Gene
    Pten (a.k.a. 2310035O07Rik, A130070J02Rik, B430203M17Rik, MMAC1, PTENbeta, TEP1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV Flag dPDZ-PTEN (1-400) was a gift from Hong Wu (Addgene plasmid # 22232 ; http://n2t.net/addgene:22232 ; RRID:Addgene_22232)

  • For your References section:

    Analysis of PTEN complex assembly and identification of hnRNP C as a component of the PTEN-associated complex. Mosessian S, Avliyakulov NK, Mulholland DJ, Boontheung P, Loo JA, Wu H. J Biol Chem. 2009 Sep 9. ():. 10.1074/jbc.M109.027995 PubMed 19740742
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