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Addgene

GFP-giantin-ATG_donor
(Plasmid #222316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP and homology arms for giantin/GOLGB1
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-giantin-ATG_donor was a gift from Franck Perez (Addgene plasmid # 222316 ; http://n2t.net/addgene:222316 ; RRID:Addgene_222316)

  • For your References section:

    Microtubule-independent secretion requires functional maturation of Golgi elements. Fourriere L, Divoux S, Roceri M, Perez F, Boncompain G. J Cell Sci. 2016 Sep 1;129(17):3238-50. doi: 10.1242/jcs.188870. Epub 2016 Jul 13. 10.1242/jcs.188870 PubMed 27411366
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