p150-CC1-tagBFP
(Plasmid
#222314)
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PurposeInactivates dynein function.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCoiled-coil domain of p150glued
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Alt nameDCTN1
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Alt namep150glued
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SpeciesH. sapiens (human)
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Entrez GeneDCTN1 (a.k.a. DAP-150, DP-150, P135)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p150-CC1-tagBFP was a gift from Franck Perez (Addgene plasmid # 222314 ; http://n2t.net/addgene:222314 ; RRID:Addgene_222314) -
For your References section:
Microtubule-independent secretion requires functional maturation of Golgi elements. Fourriere L, Divoux S, Roceri M, Perez F, Boncompain G. J Cell Sci. 2016 Sep 1;129(17):3238-50. doi: 10.1242/jcs.188870. Epub 2016 Jul 13. 10.1242/jcs.188870 PubMed 27411366