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Addgene

Str-KDEL_SBP-EGFP-CCR5-Cys3A
(Plasmid #222313)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222313 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES-neo3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP
  • Alt name
    CCR5
  • Species
    H. sapiens (human)
  • Mutation
    C321A, C323A, C324A
  • Entrez Gene
    CCR5 (a.k.a. CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_SBP-EGFP-CCR5-Cys3A was a gift from Franck Perez (Addgene plasmid # 222313 ; http://n2t.net/addgene:222313 ; RRID:Addgene_222313)

  • For your References section:

    Targeting CCR5 trafficking to inhibit HIV-1 infection. Boncompain G, Herit F, Tessier S, Lescure A, Del Nery E, Gestraud P, Staropoli I, Fukata Y, Fukata M, Brelot A, Niedergang F, Perez F. Sci Adv. 2019 Oct 16;5(10):eaax0821. doi: 10.1126/sciadv.aax0821. eCollection 2019 Oct. 10.1126/sciadv.aax0821 PubMed 31663020
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