Str-KDEL_SBP-EGFP-PLAP
(Plasmid
#222308)
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PurposeSynchronize the trafficking of PLAP from the ER.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES-neo3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStreptavidin-KDEL and PLAP fused to SBP-EGFP
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Alt nameALPP
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Alt namePLAP
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SpeciesH. sapiens (human)
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Mutationsignal peptide removed
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Entrez GeneALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Str-KDEL_SBP-EGFP-PLAP was a gift from Franck Perez (Addgene plasmid # 222308 ; http://n2t.net/addgene:222308 ; RRID:Addgene_222308) -
For your References section:
RAB6 and microtubules restrict protein secretion to focal adhesions. Fourriere L, Kasri A, Gareil N, Bardin S, Bousquet H, Pereira D, Perez F, Goud B, Boncompain G, Miserey-Lenkei S. J Cell Biol. 2019 Jul 1;218(7):2215-2231. doi: 10.1083/jcb.201805002. Epub 2019 May 29. 10.1083/jcb.201805002 PubMed 31142554