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Addgene

Str-KDEL_SBP-EGFP-PLAP
(Plasmid #222308)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222308 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES-neo3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-KDEL and PLAP fused to SBP-EGFP
  • Alt name
    ALPP
  • Alt name
    PLAP
  • Species
    H. sapiens (human)
  • Mutation
    signal peptide removed
  • Entrez Gene
    ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Str-KDEL_SBP-EGFP-PLAP was a gift from Franck Perez (Addgene plasmid # 222308 ; http://n2t.net/addgene:222308 ; RRID:Addgene_222308)
  • For your References section:

    RAB6 and microtubules restrict protein secretion to focal adhesions. Fourriere L, Kasri A, Gareil N, Bardin S, Bousquet H, Pereira D, Perez F, Goud B, Boncompain G, Miserey-Lenkei S. J Cell Biol. 2019 Jul 1;218(7):2215-2231. doi: 10.1083/jcb.201805002. Epub 2019 May 29. 10.1083/jcb.201805002 PubMed 31142554