pBIG1a_StrepII-Hook2_FTS_FHIP2A
(Plasmid
#222302)
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PurposeCo-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 222302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBig1a
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameHOOK2
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SpeciesH. sapiens (human)
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Insert Size (bp)2157
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Entrez GeneHOOK2 (a.k.a. HK2)
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Tag
/ Fusion Protein
- StrepII-Psc (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameFTS
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SpeciesH. sapiens (human)
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Insert Size (bp)876
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Entrez GeneAKTIP (a.k.a. FT1, FTS)
Gene/Insert 3
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Gene/Insert nameFHIP2A
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SpeciesH. sapiens (human)
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Insert Size (bp)2295
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Entrez GeneFHIP2A (a.k.a. FAM160B1, KIAA1600, bA106M7.3)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBIG1a_StrepII-Hook2_FTS_FHIP2A was a gift from Andrew Carter (Addgene plasmid # 222302 ; http://n2t.net/addgene:222302 ; RRID:Addgene_222302)