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MSCVneo-HA-ER-Hoxb8
(Plasmid #222291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222291 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCVneo
  • Backbone manufacturer
    Takeda
  • Backbone size w/o insert (bp) 6436
  • Total vector size (bp) 8155
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER-Hoxb8
  • Alt name
    Human estrogen receptor alpha hormone binding domain fused to murine hoxb8
  • Alt name
    The hormone binding domain = amino acids 282-595
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    1677
  • Mutation
    The estrogen receptor hormone binding domain contains the G400V point mutation
  • GenBank ID
    2099, 15416
  • Entrez Gene
    ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
  • Entrez Gene
    Hoxb8 (a.k.a. Hox-2.4)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer None
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCVneo-HA-ER-Hoxb8 was a gift from Mark Kamps & David Sykes (Addgene plasmid # 222291 ; http://n2t.net/addgene:222291 ; RRID:Addgene_222291)

  • For your References section:

    Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8. Wang GG, Calvo KR, Pasillas MP, Sykes DB, Hacker H, Kamps MP. Nat Methods. 2006 Apr;3(4):287-93. 10.1038/nmeth865 PubMed 16554834
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