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Addgene

pEGFPC1-EPSIN 1
(Plasmid #22228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Epsin 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1800
  • Mutation
    please see depositor comment below
  • GenBank ID
    AF018261
  • Entrez Gene
    Epn1
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primere
  • 3′ sequencing primer EGFP-C Sequencing Primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Addgene's quality control sequence found the EPSIN1 gene has an insertion of threonine after aa 821 and since there is no stop codon, ESPIN1 contains the additional c terminal residues VDGTAGPGSTGSR. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-EPSIN 1 was a gift from Pietro De Camilli (Addgene plasmid # 22228 ; http://n2t.net/addgene:22228 ; RRID:Addgene_22228)

  • For your References section:

    Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis. Chen H, Fre S, Slepnev VI, Capua MR, Takei K, Butler MH, Di Fiore PP, De Camilli P. Nature. 1998 Aug 20. 394(6695):793-7. 10.1038/29555 PubMed 9723620
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